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Maintaining adequate levels of these vitamins is critical to the proper function of DNMTs and the maintenance of DNA methylation patterns (Guant et al

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Immunofluorescence (IF) Monolayers grown on glass coverslips were fixed in 4% paraformaldehyde (PFA) for 15 min and permeabilised with blocking solution (PBS supplemented with 0.1% saponin, 0.5% BSA and 50 mM NH 4 Cl) for 30 min