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We adjusted the pooling ratio for a production-scale closure that we termed DOSEDO_v2 (Supplementary Methods 2.5)

Working with a complete setup minimizes contamination risk and ensures a smooth process

For blood collected for PCR analysis, the tube containing EDTA as an anticoagulant and frozen at -20C was used

To increase comparability in the second experiment, cells of both conditions were seeded in a 15 cm dish at low density to allow single clone colony formation