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The gut microbiota composition has been shown to influence the immune responses of distant organs, including lungs, through the systemic dissemination of metabolites such as short chain fatty acids (SCFAs), or through the direct seeding of bacteria from the gut to the lungs by gastro-oesophageal reflux and microaspiration

Not everyone does, but labs can be helpful especially if fatigue or suspected deficiencies are part of the story

This vial arrives lyophilised (freeze-dried powder) and requires reconstitution with bacteriostatic water before use in research

Whenever required, the stocks were thawed and diluted 1:500 in 7H9 (Difco, BBL) medium supplemented with Oleic acid, Albumin, Dextrose, Salt (NaCl) (OADS), and 0.01% Tween 80