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The cells were further incubated on a shaker at room temperature (RT) for 30 min and absorbance was measured at 490 nm on a microplate reader (Thermo Scientific)

In scientific literature, subcutaneous administration once daily is most commonly described. The experimental doses used typically range between 250500 g (0.250.5 mg) daily

Anecdotally, due to its purported regenerative capabilities, it has also earned the nickname "Wolverine" peptide, a reference to the comic book character known for rapid healing