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The value of this variable, called Z B , can be calculated for each titration point according to Equation (1): (1) In Equation (1), H is the total concentration of protons in the solution (calculated according to Equation (2) below), h is the free concentration of protons in the solution (calculated according to Equation (3) below), [OH ] is the concentration of hydroxide ions in solution and B is the total concentration of DHBA in the solution (calculated according to Equation (4) below)

Wash twice with 2.5 mL each of Wash Buffer

Guizhen Zhao, University of Michigan, United States Updates Copyright 2022 Zhou, Cheng, He, Xie, Xu, Xu and Huang