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In addition, baicalin also reduced KLF6, a fibrogenic nuclear protein that is directly targeted by miR122 (Zhang et al., 2016)

doi: 10.1016/j.heliyon.2021.e06734 153 NguyenNQNguyenMTNguyenVTLeVMTrieuLHLeXTet al

Extracted proteins were denatured in 100 L of 6 M urea, reduced with 10 mM dithiothreitol (DTT) at room temperature for 60 min, and alkylated with 15 mM iodoacetamide in the dark for 60 min