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Three things affect the availability of BH4: How much is made ( de novo synthesis) How much is needed and used in reactions How much of it is recycled or regenerated (salvage route) 1

After treatment, the cells were fixed with 4% paraformaldehyde for 30 min at room temperature, washed with Tris-phosphate buffer and incubated overnight at room temperature with the following antibodies Anti-GPX4 (1:100), ani-3F3-FMA (1:100) and anti-Drp1 (1:100) or Anti-vimentin (1:100) overnight

Organic small molecule-based photo-thermal agents for cancer therapy: Design strategies from single-molecule optimization to synergistic enhancement