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The libraries were quantified to be pooled equimolarly and sequenced on an Illumina NextSeq 2000 with V3 chemistry pair-end 150 cycles to achieve sequencing depth ~100 million reads per sample

A two-tailed Students t-test was used to compare two groups and a one-way ANOVA and Bonferroni Post-hoc Analysis were used for comparing multiple groups

Middle childhood thus appears to represent a critical period in social development (, , ), raising the question of whether alterations to the adaptive onset and completion of this stage, in the context of its higher level of social challenges, can represent important causes of variation in the symptoms of autism, especially with regard to later-diagnosed forms of this condition