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Mol Cell (2019) 73:429445.e7

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Answer: Frequency depends on your starting levels and goals

In brief, 1 10 5 spleen Ter119+ cells were pelleted in PBS, resuspended in 30 l of Buffer CM and incubated at 24 C for 1 h, with occasional vortex

However, a Cochrane review concluded that benefits are more evident in children over 6 months old and in populations with high risk of zinc deficiency, with limited evidence for benefit in well-nourished or younger children (Lazzerini and Wanzira, 2016)