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In order to get rid of contaminating metal ions in the buffer, the mobile phase was eluted through the Chelex100 Chelating Ion Exchange resin (Sigma, Merck KGaA, Darmstadt, Germany) prior to liquid chromatographic separation

137 Blood Proteins as Antioxidants Human blood proteins are vulnerable to oxidative damage because free radicals can cause alterations in the electrical charge of proteins, induce cross-linking of proteins, and increase their susceptibility to proteolysis

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Vitamin B12 for dogs is involved in several metabolic processes and aids in many biological functions