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Therefore, we optimized our search strategy by categorizing the botanical drugs into metabolites such as flavonoids, polyphenols, terpenoids, alkaloids, saponins, and anthraquinones, and combining these with more specific keywords like ALD, alcohol-related liver disease, alcoholic liver disease, oxidative stress, and ROS to improve the search accuracy

It was also pointed out that limited proteolysis represents a convenient and accurate method for determining the ratio of denaturation quantitative determination of denatured and native statesof partially denatured proteins (Okunuki et al., 1956)

Peptide concentration was determined by weight and confirmed by NMR, and protein concentrations by A280 measurements

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