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Importantly, this work also revealed the synaptic and behavioral abnormalities in these models of ASD [172, 173, 175] that were reversed by the selective nNOS inhibitor 7-nitroindasole [172, 173]

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The biofilm suspension was then serially diluted in 1 PBS and 100 ml aliquots were spread on TSB plates and incubated for 24 h at 37C