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Fast axonal transport Rapid transport of vesicles, mitochondria and other cargo along axonal microtubules

10.1083/jcb.111.5.2097 121 XieC.WeiW.ZhangT.DirschO.DahmenU

HETEROLOGOUS EXPRESSION OF AsGGT1, AsGGT2, AND AsGGT3 IN YEAST The coding regions of AsGGT1 , AsGGT2 , and AsGGT3 were amplified by PCR using the cloned cDNA fragments described above, KOD plus DNA polymerase (Toyobo), and the following gene-specific primers: AsGGT1-FKpn3A (5- GGTACC AAAATGAACCAAATGGCGCCGGCTTC-3) and AsGGT1-stop-RXh (5- CTCGAG CTATACACAAGCAGGACTTC CATC-3) for AsGGT1

The ion gradients established by Na, K-ATPase provide the driving force for other processes such as sodium-calcium exchange, sodium-bicarbonate cotransport,143 and amino acid transport.144 The mechanistic details of the sodium pump are well documented.145 The combination of passive diffusion and anteriorly located energy-dependent transporters has been described as a pump-leak system146 (Fig