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On the respective days of inoculation, the inocula preserved in liquid nitrogen in multiple Eppendorf tubes were thawed, and 25 microliters of the inoculum were mixed with 1 mL of the refrigerated inoculation solution in a sterile environment

We divided the patients into two groups based on concomitant diagnosis / procedure of NAFLD/NASH based on ICD-10 codes, defined as having diagnosis codes of fatty liver not elsewhere classified (K76.0), NASH (K75.81), hepatic fibrosis (K74.0), or cryptogenic liver disease with or without cirrhosis (K76.9 and K74.6, respectively)