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Several studies chose to validate their defined signatures in a secondary dataset and noted improved prediction of outcomes when metabolic biomarkers were used with protein biomarkers (S100b, UCH-L1, GFAP, etc.) [30, 31]

To measure systemic oxidative stress, freshly isolated 5 10 5 PBMCs were diluted 1:1 with a 10 mM 5,5dithiobis 2-nitrobenzoic acid (DTNB) solution and kept frozen at 80C until use

Subsequently, the groups investigated the antidepressant mechanism of Tiansi liquid on a hydrocortisone-induced depression rat model ( Tryptophan metabolism is implicated in several hypotheses regarding the pathogenesis of depression