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After the geometry optimizations, the vertical excitation and emission energies are calculated at the S 0 and S 1 equilibrium structures (denoted as (S 0 ) min and (S 1 ) min ), respectively

We identified R241 and S9 as critical residues required to maintain this inhibitory mechanism, the latter of which is preferentially phosphorylated in livers of old mice 49 , thus implicating it as a phosphoregulatory switch