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Except your dose was supposed to increase to 4mg at week 5, which means 80 units from the same vial

NNMT uses the universal methyl donor SAM and produces increased concentrations of 1-MNA, which is further oxidized to 2-PY and 4-PY and eventually excreted in the urine (90)

For the LEfSe analysis, an alpha of 0.05 was set for the KruskalWallis tests among classes, and per-sample normalization was performed to bring the sum of the values to 1 M