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DOI: Received: Accepted: Published: Publisher Name: Springer, Cham Print ISBN: 978-3-031-08115-6 Online ISBN: 978-3-031-08115-6 eBook Packages: Living Reference MedicineReference Module Medicine

As discussed in Bioprocess Design, a CO 2 -bicarbonate system can be achieved by exogenous addition of 5-10% gaseous CO 2 (often delivered in bioreactor systems via sparging), which reaches equilibrium in solution with bicarbonate ions, forming a natural buffer system