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Step 3: Push the plunger in to add 10 mL of air to the vial and then draw out 10 mL of bacteriostatic water by slowly pulling the plunger back out

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Being honest about that gap is not a weakness of this guide it is the accurate picture, and it is why every dosing figure here is framed as a research protocol rather than a recommendation

Subsequently, for these data as well as the alpha diversity analyses, each treatment group (within each phylum or genus for the relative abundance data) was analyzed for normal distribution (ShapiroWilk test) and the data across groups were tested for homoscedasticity (BrownForsythe test)