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At the end of the study, the mice were killed by decapitation after complete anesthesia

Biochemical investigations, in silico study and targeted delivery aspects of plant phytosterols: -sitosterol

For example, HA and IR have been shown to trigger ferroptosis in the pregnant uterus and placental tissue ( 4 Gut microbiota: a potential regulatory hub for ferroptosis in PCOS 4.1 Gut microbiota dysbiosis and host iron metabolism dysregulation The gut microbiota comprises symbiotic microorganisms that colonize the human intestinal tract and interact with the ovaries through microbial metabolites, immune signaling, and neuroendocrine pathways

This indicates that they have the ability to replace the initial requirement for serum trophic factors in neuronal cultures