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For example, human induced pluripotent stem cell (iPS cell) astrocyte studies indicate that PSEN1 mutations enhance cytokine signaling via disrupted intramembrane proteolysis, lowering the threshold for astrocyte reactivity and predisposing cells to exaggerated inflammatory responses to modest stimuli 79

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T reg differentiation For regulatory Tcell (T reg ) differentiation, CD4 + T cells were incubated for 5 days using TexMACS TM medium (# 130097196, Miltenyi Biotec, Bergisch Gladbach, Germany), stimulated with T Cell TransAct TM human (Miltenyi Biotec, Bergisch Gladbach, Germany) and supplemented with 5 ng/ml TGF1, 1 g/ml antiIFN, 1 g/ml antiIL4, 100 U/ml IL2 (#130 095067 | #130 095743 | # 130095709 | #130 097 742, Miltenyi Biotec, Bergisch Gladbach, Germany), and 10 mM of retinoic acid ( #R2625