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This means: Cleavage leaves a completely native N-terminus no extra residues Extremely specificno off-target cleavage Efficienttypically complete cleavage in 12 hours at room temperature The cleaved SUMO tag can be removed by reverse Ni-NTA (if His6-SUMO was used) Affinity purification: Typically used as His6-SUMO, relying on the His6 tag for Ni-NTA purification

You would think that kind of a human being or that kind of an intelligent creature would be able to accomplish way more