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For GST-fused CENP-C fragments, we used the previously described pGEX-6P-1_ggCENP-C 677-864 and pGEX-6P-1_hsCENP-C 760-943 (Hara et al, 2023)

Muscle tissue burns more calories at rest than fat tissue, so preserving your existing muscle helps maintain metabolic rate even as total body weight decreases

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The laboratory animals were housed in PC cages in conventional laboratory conditions at a temperature of 22.4 C, a relative humidity of 4070%, and a noise level of 60 dB