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Description

The rats were group-housed (34 rats per cage) in ventilated cages in a humidity- and temperature-controlled room (temperature, 21 2 C

After 72 h, cells were fixed in 4% PFA for 45 min and then stained with DAPI for 15 min

The PLB-985 neutrophils were not treated with NAC, but we included in this assay the gp91 phox KO-PLB-985 cells (hereafter referred to as PLB-985- nox2 KO) [22]