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For BM, 3-fold excess acetonitrile: methanol (3:1) was added for protein removal

The ominous octet (Defronzo model) includes: decreased muscle glucose uptake, increased hepatic glucose output, impaired incretin effect, alpha-cell hyperglucagonemia, increased renal glucose reabsorption (SGLT-2 upregulation), increased lipolysis (adipose), decreased central satiety signaling, and decreased beta-cell insulin secretion

Glutamine functions as a precursor for neurotransmitter synthesis and helps maintain acid-base balance within the kidneys

The primary phytohormones involved in HS reactions are strigolactones, gibberellins, cytokinins, ethylene, jasmonates, melatonin, salicylic acid (SA), abscisic acid (ABA), auxin Gamma-aminobutyric acid (GABA) and brassinosteroids (BRs)