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After dilution, an estrogen isotope and internal standard mixture with a final concentration of 50 ng/ml were added to the diluted urine sample

Monitoring and adjusting treatment plans is crucial to ensure patient safety and optimal outcomes

The following phenomena were observed based on TEM analysis in group B: a large number of vacuoles in the cytoplasm, obvious oedema and cavitation of organelles, increased levels of abnormal lipids, rupture of the nuclear membrane of interstitial cells, mitochondrial oedema, partial absence of cytoplasm, increased nuclear heterochromatin, expansion of endoplasmic reticulum, obvious damage to the spermatogonia membrane, a few vacuoles near the membrane, endoplasm of primary spermatocytes, insignificant swelling of the endoplasmic reticulum, the lack of a defined cell membrane, increased nuclear heterochromatin and the formation of large vacuoles next to secondary spermatocytes

At first, the enzyme named -glutamylcysteine synthase (-GCS) also known as glutamate cysteine ligase (GCL) links cysteine and glutamate to form -glutamylcysteine