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Briefly, 400 m slices were cultured on (Milipore, Billerica, Mass.) for 3 days after which they were biolistically transfected with tomato fluorescent protein (TFP) using a Helios Gene Gun (BioRad, Hercules, Calif.), according to the manufacturer's protocol, to visualize dendritic arbors

Future studies will focus on refining this technology to enhance precision in gene expression and explore its implications for clinical translation

Hence, the development of an efficient drug delivery system has the potential to facilitate a continuous and regulated release of therapeutic substances, thereby fostering recovery from the secondary damage to the brain following TBI (Mohammed et al., 2023)

CXCL4 is one of the most consequential chemokines in the CXC subfamily, and there is evidence that the heterodimer CXCL4-CCL5 has appertained to the process of brain injury, and the addition of inhibitors can significantly enhance neurological function in mice (Vogel et al., 2014