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All positively stained cells were counted and the % Nissl positive neuron was calculated by dividing the number of positive neurons by the total number of neurons in each cell [1]

While the precise mechanisms continue to be investigated, findings suggest that DSIP plays an important role in sleep-associated biological processes that contribute to mental restoration, focus, and long-term brain function

This is particularly significant in the context of wound healing, where senescent cells play a supportive role in regenerative processes