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One plate was used for each point (day) of the assay and each condition (with or without IGF1) in triplicate, plus an additional plate that was used to determine the initial number of cells (time zero plate)

G6PDi1 Is a Potent Inhibitor of G6PDH and of Pentose Phosphate PathwayDependent Metabolic Processes in Cultured Primary Astrocytes. Neurochemical Research 48: 31773189