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When B12 levels are equivocal, additional testing with methylmalonic acid (MMA) and homocysteine levels can provide greater diagnostic accuracy, as both are elevated in true B12 deficiency

The three formulas the calculator uses: Concentration (mg/mL) = vial mg bacteriostatic water mL Units to draw = (target mcg (vial mg 1000)) water mL 100 Doses per vial = (vial mg 1000) target mcg, rounded down A 5 mg vial reconstituted with 2 mL of bacteriostatic water is 2.5 mg/mL, or 25 mcg per unit

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Extreme restriction is unnecessary and often counterproductive, causing metabolic slowdown that persists beyond the protocol