Adv Protein Chem Struct Biol 86:143 Yin G, Garces ED, Yang J, Zhang J, Tran C, Steiner AR, Roos C, Bajad S, Hudak S, Penta K, Zawada J, Pollitt S, Murray CJ (2012) Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system
Overall, PEG treatment significantly boosts phenylpropanoid and flavonoid metabolism in LY46, reflecting a stronger metabolic response than the relatively slowly and quite changes in HGN
rabbit anti-phospho-VEGFR2 Tyr1175 (#2478, 1: 800), rabbit anti-phospho-p38MAPK Thr180/Tyr182 (#4631 S, 1: 1,000), rabbit anti-p38MAPK (#9212, 1: 1,000), rabbit anti-STAT1 (#9172, 1: 500), and rabbit anti-phospho-STAT1 Ser727 (#8826, 1: 500) antibodies from Cell Signaling Technology (Danvers, MA, USA)
Reactions were incubated at room temperature for 20 min prior to reading at 490 nm using an Infinite F200 Pro plate reader (Tecan)