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doi: 10.1074/jbc.274.30.20847 118 HainsPGMizdrakJStreeteIMJamieJFTruscottRJW

The upper polar phase (200 l) was transferred into GC vials and evaporated to complete dryness at 4 C in a vacuum centrifuge (Labconco Corporation) for at least 3 h

Following centrifugation twice (10,000 x g , 15 min, room temperature) with the sample transferred to a new tube between centrifugation steps, GSSG levels were measured spectrophotometrically as outlined above